Ox-100C Tri-Gas Incubator

Ox-100C Tri-Gas Incubator

Oxygen concentration and carbon dioxide concentration in cell culture can be controlled by Ox-100C Tri-Gas Incubator, also known as cell hypoxia experiment system. Ox-100C Tri-Gas Incubator can be used to simulate physiological hypoxia in vivo, so that in vitro research, the results are closer to the real level in vivo. It can also be used to simulate the pathological state of organisms in specific hypoxic state, to study related pathological and physiological mechanisms and hypoxia diseases.

Ox-100C Tri-Gas Incubator consists of a gas controller and a portable cell hypoxic chamber. During the application, the cell hypoxic chamber is put into a conventional experimental incubator to maintain a constant temperature. The oxygen concentration and carbon dioxide concentration in the cell hypoxic chamber could be controlled by an external controller.The cell chamber has a built-in water tray to maintain the necessary humidity for cell culture. Therefore, Ox-100C Tri-Gas Incubator is an ideal solution to upgrade an existing conventional incubator with additional low oxygen concentration control function economically.

Comprehensive monitoring function

Ox-100C Tri-Gas Incubator are capable of monitoring the following comprehensive indicators, including temperature, humidity, oxygen, carbon dioxide sensors related data, and can be retrieved using computer.

Precise Control

Closed-loop feedback control: the system monitors the environment in the low-oxygen chamber in real time to acquire real-time feedback control, which in turn improves the accuracy of the experimental low-oxygen data and avoids the inconsistency between the controlled concentration output and the concentration in the low-oxygen chamber.

O₂ concentration control range: 0.1-20%, Control Precision:0.1%

CO₂ concentration control range: 0-20%, Control Precision: 0.1%

OX-100C with higher accuracy is recommended for sole hypoxic experiments. For high oxygen experiments, OX-100CHE with oxygen control range of 0.1-100% is recommended.

User-friendly Design

All settings are controlled by the touch screen of the host computer with user-friendly design and ease of operation.

Cell Hypoxic Chamber

1. External Dimension: 360×300×150mm, other sizes can be customized
2. Transparent PMMA material (Acrylic)
3. Standard double layer cell culture tray, stainless steel, removable
4. Built-in water tray

Upgradable Functions (Optional)

1. Complete Independent Usage with Additional Temperature Control Function: Temperature Control Range: Room Temperature-50℃, Temperature Control Precision 0.1℃
2. Multichannel: 3-channel controller is provided to simultaneously control 3 hypoxic chambers for parallel control experiments at different concentrations
3. Dissolved oxygen probe can also be provided to monitor the oxygen content in the cell culture medium in real time.
4. Tow-Int Tech can provide a variety of different gas controllers for O₂, CO₂, NO, CO, O₃, SO₂, and automobile exhaust studies.

Schematic Diagram

Schematic diagram of Ox-100C Tri-Gas Incubator

The system can simultaneously control 3 hypoxic chambers and conduct parallel control experiments with different concentrations.

Note: Conventional Incubator and gas cylinders are to be purchased separately.

Product Features and Parameters:

1. Simulating an in vivo hypoxic environment for cells culture in vitro.

2. Auto-mixing of gases according to the preset gas concentration, ensuring the gas concentration requirement under the       condition of constant oxygen concentration experiment. Gas-mixing outside the chamber is avoided to ensure       the accuracy of gas concentration and reduce gases waste.

3. The chamber is made of imported transparent PMMA material, which is strong and durable.

4. 7 inches touch screen control, user-friendly interface, ease of operation;

5. Monitoring parameters: temperature, humidity, oxygen, carbon dioxide concentration;

6. Control parameters: temperature (selected), humidity, oxygen, carbon dioxide

7. O₂ Concentration Detection: imported O₂ detector

Control Range: 0.1-20.0%vol

Precision: 0.1%

Good Linearity

Accurate Detection

Extended Optional Range 0-100% ;

8. CO₂ Concentration Detection: Imported Detector

Control Range: 0 ~ 20%;

9. Temperature Detection: Imported High-precision Platinum Resistance Temperature Sensor

10. Humidity Sensor: Monitoring the humidity in the chamber to guarantee a humid environment for cell growth.

11. The dynamic change curve of the indicators can be viewed in real time, and also changes of temperature, humidity, O₂ and CO₂ concentration.

12. Data can be saved and archived in the computer.

13. Auto-calibration of gas concentration: Quick calibration of the sensors by controller

14. Gas mixing and circulating devices which ensure the uniform in-chamber gas concentration.

15. High performance electromagnetic valve, stable performance, long service life.

16. The built-in stainless steel porous board is used for cell culture dish, culture flask, 96-pore board, etc.

17. Equipped with a water tray.

Applicable Areas

• Cell growth: increased proliferation of stem cells and progenitor cells to repair trauma;
• Gene expression: studies have shown that 5% of gene expression in the genome is regulated by oxygen concentration
• Cell morphology and differentiation: regulate cell differentiation and influence cell morphology;
• Tissue engineering: high vascularized tissue grows better at higher oxygen concentration;Non-vascular tissue, such as articular cartilage, performed better at low oxygen concentrations;
• Neurobiology: hypoxia preconditioning can protect neurons from subsequent severe hypoxia or other fatal stress.
• In vitro experiments on cardiovascular diseases: studies have shown that in vitro hypoxic culture can accelerate the spread of cells, promote the degradation of extra-cellular matrix and cell migration, and thus promote angiogenesis and endothelial differentiation.
• Cancer and tumor therapy research: human bone marrow mesenchymal stem cells have been shown to promote tumor angiogenesis.

Ox-100C Tri-Gas Incubators Models：

Our company can provide 3Q qualification to assist customers to qualify the system in all aspects.